Identification, cloning, and expression of the Scytalidium acidophilum XYL1 gene encoding for an acidophilic xylanase.
نویسندگان
چکیده
We cloned XYL1, a Scytalidium acidophilum gene encoding for an acidophilic family 11 xylanase. The XYL1p protein was expressed in Pichia pastoris using the pPICZalphaA expression plasmid. The secreted protein was purified by TAXI affinity column chromatography. The purified XYL1p showed an optimum activity at pH 3.2 and 56 degrees C. The Michaelis-Menten constants were determined.
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Acidophilic adaptation of family 11 endo-beta-1,4-xylanases: modeling and mutational analysis.
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ورودعنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 70 1 شماره
صفحات -
تاریخ انتشار 2006